Journal: STAR Protocols
Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays
doi: 10.1016/j.xpro.2026.104361
Figure Lengend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.
Techniques: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence